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th2 cytokines balf  (R&D Systems)


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    R&D Systems th2 cytokines balf
    Proposed anti-inflammatory mechanism of black ginseng extract (BGE) in both phorbol 12-myristate 13-acetate plus ionomycin-stimulated EL4 cells and ovalbumin-sensitized mice with allergic airway inflammation. PMA, phorbol 12-myristate 13-acetate; IL, interleukin; PKCθ, protein kinase C theta; BGE, black ginseng extract; NF-κB, nuclear factor kappa B; NFAT, nuclear factor of activated T cells; STAT6, signal transducer and activator of transcription 6; GATA3, GATA binding protein 3; <t>Th2,</t> T helper 2; IgE, immunoglobulin E; AHR, airway hyperresponsiveness.
    Th2 Cytokines Balf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/th2+cytokines+balf/pmc10418634-152-3-7?v=R%26D+Systems
    Average 90 stars, based on 1 article reviews
    th2 cytokines balf - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway"

    Article Title: Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms241511970

    Proposed anti-inflammatory mechanism of black ginseng extract (BGE) in both phorbol 12-myristate 13-acetate plus ionomycin-stimulated EL4 cells and ovalbumin-sensitized mice with allergic airway inflammation. PMA, phorbol 12-myristate 13-acetate; IL, interleukin; PKCθ, protein kinase C theta; BGE, black ginseng extract; NF-κB, nuclear factor kappa B; NFAT, nuclear factor of activated T cells; STAT6, signal transducer and activator of transcription 6; GATA3, GATA binding protein 3; Th2, T helper 2; IgE, immunoglobulin E; AHR, airway hyperresponsiveness.
    Figure Legend Snippet: Proposed anti-inflammatory mechanism of black ginseng extract (BGE) in both phorbol 12-myristate 13-acetate plus ionomycin-stimulated EL4 cells and ovalbumin-sensitized mice with allergic airway inflammation. PMA, phorbol 12-myristate 13-acetate; IL, interleukin; PKCθ, protein kinase C theta; BGE, black ginseng extract; NF-κB, nuclear factor kappa B; NFAT, nuclear factor of activated T cells; STAT6, signal transducer and activator of transcription 6; GATA3, GATA binding protein 3; Th2, T helper 2; IgE, immunoglobulin E; AHR, airway hyperresponsiveness.

    Techniques Used: Binding Assay

    Effects of black ginseng extract (BGE) on the expression of T helper 2 cytokines in phorbol 12-myristate 13-acetate plus ionomycin (PMA/Iono)-stimulated EL4 cells. ( A ) Cell viability was measured using the cell counting kit-8 assay. ( B – D ) The secretion levels of interleukin (IL)-4, -5, and -13 were assessed using enzyme-linked immunosorbent assay kits. ( E – G ) The mRNA expression levels of Il-4 , -5 , and -13 were evaluated using quantitative real-time polymerase chain reaction. Bar graphs represent the means ± standard deviation in triplicate in one representative experiment from a total of three independent experiments. # p < 0.001 vs. negative control group (without PMA/Iono); * p < 0.05; ** p < 0.01, and *** p < 0.001 for comparison with controls, which were treated with PMA/Iono alone.
    Figure Legend Snippet: Effects of black ginseng extract (BGE) on the expression of T helper 2 cytokines in phorbol 12-myristate 13-acetate plus ionomycin (PMA/Iono)-stimulated EL4 cells. ( A ) Cell viability was measured using the cell counting kit-8 assay. ( B – D ) The secretion levels of interleukin (IL)-4, -5, and -13 were assessed using enzyme-linked immunosorbent assay kits. ( E – G ) The mRNA expression levels of Il-4 , -5 , and -13 were evaluated using quantitative real-time polymerase chain reaction. Bar graphs represent the means ± standard deviation in triplicate in one representative experiment from a total of three independent experiments. # p < 0.001 vs. negative control group (without PMA/Iono); * p < 0.05; ** p < 0.01, and *** p < 0.001 for comparison with controls, which were treated with PMA/Iono alone.

    Techniques Used: Expressing, Cell Counting, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Standard Deviation, Negative Control, Comparison



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    Proposed anti-inflammatory mechanism of black ginseng extract (BGE) in both phorbol 12-myristate 13-acetate plus ionomycin-stimulated EL4 cells and ovalbumin-sensitized mice with allergic airway inflammation. PMA, phorbol 12-myristate 13-acetate; IL, interleukin; PKCθ, protein kinase C theta; BGE, black ginseng extract; NF-κB, nuclear factor kappa B; NFAT, nuclear factor of activated T cells; STAT6, signal transducer and activator of transcription 6; GATA3, GATA binding protein 3; <t>Th2,</t> T helper 2; IgE, immunoglobulin E; AHR, airway hyperresponsiveness.
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    Image Search Results


    Proposed anti-inflammatory mechanism of black ginseng extract (BGE) in both phorbol 12-myristate 13-acetate plus ionomycin-stimulated EL4 cells and ovalbumin-sensitized mice with allergic airway inflammation. PMA, phorbol 12-myristate 13-acetate; IL, interleukin; PKCθ, protein kinase C theta; BGE, black ginseng extract; NF-κB, nuclear factor kappa B; NFAT, nuclear factor of activated T cells; STAT6, signal transducer and activator of transcription 6; GATA3, GATA binding protein 3; Th2, T helper 2; IgE, immunoglobulin E; AHR, airway hyperresponsiveness.

    Journal: International Journal of Molecular Sciences

    Article Title: Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway

    doi: 10.3390/ijms241511970

    Figure Lengend Snippet: Proposed anti-inflammatory mechanism of black ginseng extract (BGE) in both phorbol 12-myristate 13-acetate plus ionomycin-stimulated EL4 cells and ovalbumin-sensitized mice with allergic airway inflammation. PMA, phorbol 12-myristate 13-acetate; IL, interleukin; PKCθ, protein kinase C theta; BGE, black ginseng extract; NF-κB, nuclear factor kappa B; NFAT, nuclear factor of activated T cells; STAT6, signal transducer and activator of transcription 6; GATA3, GATA binding protein 3; Th2, T helper 2; IgE, immunoglobulin E; AHR, airway hyperresponsiveness.

    Article Snippet: The levels of Th2 cytokines in BALF (R&D systems, Minneapolis, MN, USA) and IgE in serum (Biolegend, San Diego, CA, USA, # 439807) were measured by enzyme-linked immunosorbent assay (ELISA) kits, following the manufacturer’s instructions.

    Techniques: Binding Assay

    Effects of black ginseng extract (BGE) on the expression of T helper 2 cytokines in phorbol 12-myristate 13-acetate plus ionomycin (PMA/Iono)-stimulated EL4 cells. ( A ) Cell viability was measured using the cell counting kit-8 assay. ( B – D ) The secretion levels of interleukin (IL)-4, -5, and -13 were assessed using enzyme-linked immunosorbent assay kits. ( E – G ) The mRNA expression levels of Il-4 , -5 , and -13 were evaluated using quantitative real-time polymerase chain reaction. Bar graphs represent the means ± standard deviation in triplicate in one representative experiment from a total of three independent experiments. # p < 0.001 vs. negative control group (without PMA/Iono); * p < 0.05; ** p < 0.01, and *** p < 0.001 for comparison with controls, which were treated with PMA/Iono alone.

    Journal: International Journal of Molecular Sciences

    Article Title: Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway

    doi: 10.3390/ijms241511970

    Figure Lengend Snippet: Effects of black ginseng extract (BGE) on the expression of T helper 2 cytokines in phorbol 12-myristate 13-acetate plus ionomycin (PMA/Iono)-stimulated EL4 cells. ( A ) Cell viability was measured using the cell counting kit-8 assay. ( B – D ) The secretion levels of interleukin (IL)-4, -5, and -13 were assessed using enzyme-linked immunosorbent assay kits. ( E – G ) The mRNA expression levels of Il-4 , -5 , and -13 were evaluated using quantitative real-time polymerase chain reaction. Bar graphs represent the means ± standard deviation in triplicate in one representative experiment from a total of three independent experiments. # p < 0.001 vs. negative control group (without PMA/Iono); * p < 0.05; ** p < 0.01, and *** p < 0.001 for comparison with controls, which were treated with PMA/Iono alone.

    Article Snippet: The levels of Th2 cytokines in BALF (R&D systems, Minneapolis, MN, USA) and IgE in serum (Biolegend, San Diego, CA, USA, # 439807) were measured by enzyme-linked immunosorbent assay (ELISA) kits, following the manufacturer’s instructions.

    Techniques: Expressing, Cell Counting, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Standard Deviation, Negative Control, Comparison